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ST-2000A Mycotoxin tester

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  • 名  稱:

    ST-2000A Mycotoxin tester
  • 型  號:

    ST-2000A
  • 品  牌:

    Shengtai Instrument
  • 0531-85919987
  • 微 信 號:

    +86 13370527859
  • +86 13370527859
  • 銷售咨詢:

    點擊這里給我發(fā)消息
  • Whatsapp/Wechat+86 133 7052 7859

產(chǎn)品詳情

ST-2000A mycotoxin analyzer is a necessary analytical instrument for aflatoxin and enzyme-linked immunosorbent assay. The principle of solid-phase enzyme-linked immunosorbent assay (ELISA), namely enzyme-linked immunosorbent assay (ELISA), is composed of this instrument and B1 kit, which can be used to determine the content of aflatoxin B1 and other mycotoxins in samples in a limited and quantitative manner (if equipped with other kits, other toxins can be detected). It is widely used in the detection of aflatoxin B1 and other mycotoxins in food, feed, oil, dairy products, drugs, beverages, wine and other products.

Performance characteristics:

1. Display operation: color LCD screen, videophone distribution board, display of whole board information, touch screen operation

2. Vibrating plate function: Level 3 vibrating plate speed, time 0-255 seconds adjustable

3. Workstation: professional enzyme marker software, which can store 500 groups of programs, 100000 sample results, and provide rich calculation modes such as absorbance, linear equation, logarithmic equation, quadratic equation, cubic equation, absorbance percentage-concentration logarithmic equation, spline function, inhibition rate, etc

Technical parameters:

Light source: DC12V 22W imported halogen lamp

Wavelength range: 400-900nm

Filter: 405, 450, 492, 630nm as standard, other wavelengths optional

Reading range: 0-4.000Abs

Resolution: 0.001Abs

Indication error: ≤± 0.01Abs

Stability: ≤± 0.003Abs

Repeatability: ≤ 0.3%

Size: 400mm  * 260mm * 200mm

Light source

DC12V 22W imported halogen lamp

Wave length range

400 - 999nm

Spectral filter

405, 450, 492, 630nm and other regular wavelength

Scope of readings

  0.000 - 4.000Abs   

Repeatability

CV≤0.3%

Stability

drift ≤±0.003Abs

Resolution

0.001Abs

Absorbance indication error

≤±0.01Abs

Size:

400mm  * 260mm * 200mm

1 Principle and application

This kit uses indirect competitive ELISA method to detect aflatoxin B1 (AFB1) in grains, feed and other samples. The kit is composed of enzyme-labeled plate pre-coated with coupling antigen, horseradish enzyme marker, antibody, standard and other matching reagents. During the test, add the standard or sample solution, the aflatoxin B1 in the sample and the enzyme plate are pre-coated with the conjugate antigen to compete for the anti-aflatoxin B1 antibody. After adding the enzyme marker, use the TMB substrate to develop the color. The sample absorbance value is negatively correlated with the content of aflatoxin B1 contained in the sample. The residual amount of aflatoxin B1 in the sample can be obtained by comparing with the standard curve.

2 Technical indicators

2.1 Kit sensitivity: 0.03ppb (ng/ml)

2.2 Reaction mode: 25 ℃, 30min~15min

2.3 Lower detection limit:

Cereals...................................................... 0.15ppb

Formula feed.......................................... 0.3 ppb

Edible oil, peanut.................................... 0.6ppb

Soy sauce, wheat and other feeds.............................. 0.3 ppb

Beer.......................................... 0.3 ppb

Wine, soy sauce, vinegar.................................... 0.15ppb

2.4 Cross reaction rate:

Aflatoxin B1 100%

2.5 Sample recovery rate:

Cereals and formula feed.............................. 85% ± 15%

Peanut.................................... 82 ± 15%

Edible oil.................................... 85 ± 15%

Soy sauce, wheat and other feeds.............................. 83 ± 15%

Beer.................................... 84 ± 15%

Wine, soy sauce, vinegar............ 87 ± 15%

3 Kit composition

Enzyme marker plate.................................... 96 holes

Standard solution (black cover): 1ml each

0ppb、0.03ppb、0.06ppb、0.12ppb、0.24ppb、0.48ppb

High standard solution: 100ppb.............................. 1ml

Enzyme marker (red cap).............................. 5.5ml

Antibody working solution (blue cap).............................. 5.5ml

Substrate solution A (white cap).............................. 6ml

Substrate liquid B (black cover).............................. 6ml

Termination solution (yellow cap).............................. 6ml

20X concentrated washing solution (white cover)..................... 40ml

Instruction manual.....................1copy

4 Required equipment and reagents

4.1 Apparatus: aflatoxin tester, printer, homogenizer, nitrogen drying device, oscillator, centrifuge, graduated pipette, balance (sensitivity 0.01g)

4.2 Micro-pipette: single-channel 20 μ l-200 μ l, 100 μ l-1000 μ l, multi-channel 300 μ l

4.3 Reagent: methanol, n-hexane, trichloromethane or dichloromethane

5 Sample pretreatment

5.1 Instructions before sample processing:

The experimental apparatus must be clean and use disposable suction head to avoid contamination and interference with the experimental results.

5.2 Solution preparation:

Solution 1: sample extract

70% methanol, i.e. V methanol: V deionized water=7:3.

5.3 Sample pretreatment steps:

5.3.1 Grain processing method

1) Weigh 2g of crushed sample into a 50ml centrifuge tube, add 5ml of sample extraction solution, shake for 5 minutes, 4000 rpm at room temperature/10 minutes of separation center;

2) Take 0.5ml of supernatant, add 0.5ml of deionized water, and mix well;

3) Take 50 μ L Analysis.

Sample dilution ratio: 5 Lower detection limit: 0.15ppb

5.3.2 Processing methods for samples with strong water absorption such as corn husk and wheat bran

1) Weigh 2g of crushed sample into a 50ml centrifuge tube, add 20ml of sample extraction solution, shake for 5 minutes, 4000 rpm at room temperature/10 minutes of separation center;

2) Take 0.5ml of supernatant, add 0.5ml of deionized water, and mix well; (For the sample with extremely high toxin content, it can be diluted with 35% methanol and then tested. For example, take 0.1ml of the mixed solution in 2) and 0.9ml of 35% methanol to mix. The final sample dilution ratio is 200, and the detection limit is 6ppb.)

3) Take 50 μ L Analysis.

Sample dilution ratio: 20 Lower detection limit: 0.6ppb

Note: Because the distribution of toxin in the sample is uneven, it is necessary to take samples from multiple points and fully mix them before taking 2g for test.

5.3.3 Processing method of formula feed

1) Weigh 2g of crushed sample into a 50ml centrifuge tube, add 10ml of sample extraction solution, shake for 5 minutes, 4000 rpm at room temperature/10 minutes of separation center;

2) Take 0.5ml of supernatant, add 0.5ml of deionized water, and mix well;

3) Take 50 μ L Analysis.

Sample dilution ratio: 10 Lower detection limit: 0.3ppb

5.3.4 Feed treatment methods of edible oil, peanut and high fat

1) Weigh 2g of crushed sample into a 50ml centrifuge tube, add 8ml of n-hexane and 10ml of sample extraction solution, shake for 5min, 4000 rpm at room temperature/10 min of separation center;

2) Remove the upper liquid, take 0.5ml of the lower liquid and add 0.5ml of deionized water, mix well, then take 0.5ml of the mixed liquid and add 0.5ml of 35% methanol, shake for 30s;

3) Take 50 μ L Analysis.

Sample dilution ratio: 20 Lower detection limit: 0.6ppb

5.3.5 Food or condiments such as sauces, wheat, biscuits, pastries, and feed processing methods such as feed concentrate

1) Weigh 2g of crushed sample into a 50ml centrifuge tube, add 10ml of sample extraction solution, shake for 5 minutes, 4000 rpm at room temperature/10 minutes of separation center;

2) Take 2ml of supernatant, add 4ml of trichloromethane (or dichloromethane), shake for 5 minutes, 4000 rpm at room temperature/10 minutes of separation center;

3) Transfer the upper liquid to another container, leave the lower liquid for standby, add another 4ml of chloroform (or dichloromethane) to the upper liquid, fully shake and mix for 5 minutes, and the room temperature is 4000 rpm/separation center for 10 minutes;

4) Remove the upper liquid, combine the lower liquid twice and fully mix;

5) Take 2ml of the combined lower liquid and blow it dry under 50-60 ℃ nitrogen;

6) Add 0.5ml of sample extract to fully dissolve the dried substance, and then add 0.5ml of deionized water to mix;

7) Take 50 μ L Analysis.

Sample dilution ratio: 10 Lower detection limit: 0.3ppb

5.3.6 Beer processing method

1) Fully stir the beer (remove CO2), take 2ml of beer sample, add 1ml of distilled water, add 7ml of methanol, and shake for 5min;

2) Take 0.5ml of mixed sample solution and add 0.5ml of deionized water to mix well;

3) Take 50 μ L Analysis.

Sample dilution ratio: 10 Lower detection limit: 0.3ppb

5.3.7 Treatment method of wine, soy sauce and vinegar

1) Take 2ml of sample, add 2ml of distilled water, add 10ml of trichloromethane (or dichloromethane), shake for 5 minutes, 4000 rpm at room temperature/10 minutes of separation center;

2) Take 1ml of the lower liquid and blow it dry under 50-60 ℃ nitrogen;

3) Add 0.5ml of sample extract to fully dissolve the dried substance, then add 0.5ml of deionized water, and mix well;

5) Take 50 μ L Analysis.

Sample dilution ratio: 5 Lower detection limit: 0.15ppb

6 Steps of ELISA

Take the required reagent out of the 4 ℃ cold storage environment and place it at room temperature for more than 30min. There may be crystals that need to be restored to room temperature to fully dissolve when the washing solution is cold storage. Each liquid reagent must be shaken before use. Take out the required number of microporous plates and frames, put the unused microporous plates into self-sealing bags, and store them at 2-8 ℃.

Before the experiment, 20 × The concentrated washing solution is diluted into working washing solution by 20 times.

6.1 Numbering: number the corresponding wells of the sample and standard in sequence, make two parallel holes for each sample and standard, and record the position of the standard hole and sample hole.


6.2 Sample adding reaction: add 50 μ l/well of standard or sample into their respective wells, then add 50 μ l/well of enzyme marker, then add 50 μ l/well of antibody working solution, seal the plate with a cover plate membrane, gently shake for 5 seconds, mix, and react at 25 ℃ for 30 minutes.

6.3 Washing: Carefully remove the cover plate membrane, dry the liquid in the hole, fully wash the hole with working washing solution 250 μ l/hole for 5 times, with an interval of 30 seconds, and pat it dry with absorbent paper (the bubbles that are not removed after the pat can be punctured with a clean gun head).

6.4 Color development: add 50 μ l of substrate solution A and 50 μ l of substrate solution B to each hole, shake gently for 5 seconds and mix well, and develop color in dark at 25 ℃ for 15 minutes.

6.5 Termination: add 50 μ l of termination solution to each hole, shake gently and mix well to terminate the reaction.

6.6 Measurement of absorbance value: use the aflatoxin tester to measure the absorbance value of each hole at 450 nm (dual wavelength 450/630 nm is recommended). The determination shall be completed within 10 minutes after the reaction is terminated

6.7 The ST-2000A automatic aflatoxin tester automatically completes the determination and automatically produces the results.

Company Introduction

Shandong Shengtai Instruments Co., Ltd. is specialized in the research and production of experimental testing instruments and industrial testing instruments. It is a professional instrument company integrating instrument research and development, manufacturing, sales and service. Its products are widely used in food, flour, grain and oil, Feed, electricity, petrochemical, industrial oil, aerospace, coal quality and other industries.

For more than 10 years, Shengtai has always been adhering to the "first-class service, customer first" as the purpose, "reasonable price, honesty and trustworthiness" as the business policy. Adhere to technical innovation, with rich knowledge of the technical team and experienced, thoughtful after-sales service team.

Shengtai instrument as one of the most large-scale and competitive enterprises in the industry, with the strength of technology research and development, professional technical knowledge and innovative design concept, in the model improvement, Significant technical breakthrough has been achieved in the development of function expansion and intelligent control system.

OUR service

After-sales service commitment, our company to sell the product after-sales service commitment: warranty period of one year, long-term technical support outside the warranty period. During the warranty period, the seller shall bear the cost of the replacement of the equipment, the freight and maintenance of the instrument; outside the warranty period, the seller shall waive the maintenance fee and charge only the cost of the basic materials and freight charges when the instrument is malfunctioning and the seller shall not charge the maintenance fee during the warranty period.


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